Fluorescence characterization of denatured proteins
نویسندگان
چکیده
منابع مشابه
Amaltheys: A fluorescence-based analyzer to assess cheese milk denatured whey proteins.
The cheese industry faces many challenges to optimize cheese yield and quality. A very precise standardization of the cheese milk is needed, which is achieved by a fine control of the process and milk composition. Thorough analysis of protein composition is important to determine the amount of protein that will be retained in the curd or lost in the whey. The fluorescence-based Amaltheys analyz...
متن کاملScaling behavior and structure of denatured proteins.
An ensemble of random-coil conformations with no persistent structures has long been accepted as the classical model of denatured proteins due to its consistency with the experimentally determined scaling of protein sizes. However, recent NMR spectroscopy studies on proteins at high chemical denaturant concentrations suggest the presence of significant amounts of native-like structures, in cont...
متن کاملHeat capacity and compactness of denatured proteins.
One of the striking results of protein thermodynamics is that the heat capacity change upon denaturation is large and positive. This change is generally ascribed to the exposure of non-polar groups to water on denaturation, in analogy to the large heat capacity change for the transfer of small non-polar molecules from hydrocarbons to water. Calculations of the heat capacity based on the exposed...
متن کاملAre denatured proteins ever random coils?
T polymer chemists, the term random coil means a chain molecule whose backbone coils randomly in three-dimensional space, with a conformation described by the Gaussian probability function (1). To protein chemists, the term random coil carries an added special meaning: the backbone conformation of every amino acid residue, described by its phi, psi pair of backbone angles, is independent of the...
متن کاملSurface characterization of proteins using multi-fractal property of heat-denatured aggregates
Multi-fractal property of heat-denatured protein aggregates (HDPA) is characteristic of its individual form. The visual similarity between digitally generated microscopic images of HDPA with that of surface-image of its individual X-ray structures in protein databank (PDB) displayed using Visual Molecular Dynamics (VMD) viewer is the basis of the study. We deigned experiments to view the fracta...
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ژورنال
عنوان ژورنال: Current Opinion in Structural Biology
سال: 2008
ISSN: 0959-440X
DOI: 10.1016/j.sbi.2008.06.008